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The unique sequence tags (i.e., barcodes) linked to each gene deletion allow the strains to be analyzed in parallel in competitive fitness assays

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Fe 2+ formed by intestinal absorption or erythrocyte degradation can be oxidized by copper cyanide to Fe 3+ , which binds to transferrin on the cell membrane and is further transported into the cell via transferrin receptor 1 (TFR1) [39]