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However, this study used only hemolytic assay in vitro to measure toxicity and lacks toxicological evaluation in vivo and thus alarming their use in clinical practice

Disadvantages are low bioavailability, poor stability, and obvious sulfur odor

Additionally, silymarin was shown in animal studies to have renoprotective effects against nephrotoxicity caused by cisplatin, adriamycin, and other chemotherapeutic agents, as indicated by decreased blood urea nitrogen, serum creatinine, and oxidative stress markers

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Prior to imaging, HepG2 cells were stained with 100 ng/mL Hoechst 33342 to visualize the nuclei