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Animals were acclimatized for one week before experimentation under controlled conditions (22 C, 12-h light/dark cycle) with food and water available ad libitum

It is well-established that disruption of the hepatic fatty acid oxidation, lipogenesis, and insulin signaling pathways contributes to hepatic steatosis

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Data were acquired in counting mode at a defocus range of 1.2 m to 1.8 m, yielding a pixel size of 0.85 at the specimen level

On the contrary, GPX4 activation suppressed ferroptosis and inflammatory responses via blunting arachidonic acid (AA) oxidation and NF-B pathway activation, accompanying by decreased ROS generation [57]