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The rate constant for the fusion construct with dithiol PfGrx C88S was about three times smaller and decreased again fivefold for monothiol PfGrx C32S/C88S (Fig
doi: 10.1016/j.cbi.2021.109625 59 DzydzanOBrodyakIStrugaa-DanakPStrachAKucharskaAZGabrielskaJet al
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At the end of all incubations, the hECs were washed three times with ice-cold PBS containing 0.1% BSA, once with acid stripping buffer (glycine 50 mM, NaCl 100 mM, pH 3) to remove the non-internalized, but cell surface-attached nanocarriers, and finally with PBS