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10.1016/j.ab.2021.114330 7

DNA was extracted with an extraction buffer (Sigma, E7526) and tissue prep solution (Sigma, T3073), heated for 10 min and 3 min at 55 C and 100 C, respectively, then neutralized with a neutralization solution (Sigma, N3910)

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Upon activation, proapoptotic proteins form pore complexes on the mitochondrial membrane, resulting in the loss of the mitochondrial membrane potential and the release of apoptotic factors such as cytochrome C, thereby activating the caspase-9/3 cascade [46]