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Retatrutide increased cAMP in these receptor-transfected cells with affinities of (in nanomolar concentrations): 5.8, 0.06, and 0.78 at GCGR, GIPR, and GLP1-R, respectively (Coskun et al

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Nippon Gene, Tokyo, Japan) by using the lithium acetate method ( GAL1 promoter on pYES2, and cultured at 28C for 1 days

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