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Live cells were then washed with phosphate-buffered saline 1, incubated for 30 min with 5 M JC-1 (37 C, 5% CO 2 ), washed with PBS 1, trypsinized, and recovered in a final volume of 300 L of PBS

Similarly, the actions of GLP-1 on inhibition of gastric emptying are also preserved in subjects with poorly controlled type 2 diabetes

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