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Target gene expression was calculated using QuantStudio v1.3 (Applied Biosystems), normalized to the ACTB housekeeping gene using the C T method, and adjusted relative to the control condition

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HUBERMAN: And in addition to wanting to teach you about some of the biology of these peptides and how they work and what they're designed to do, as well as some of their potential therapeutic benefits under the right conditions, again, working with a really good board-certified physician and making sure that the sourcing is really clean

Step 5: Inspect the solution

The honest position is that the systemic pharmacology of injectable GHK-Cu is reasoned rather than measured, and any number one might attach to a dose would therefore be extrapolation resting on assumptions the data have not tested