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One of the significant advantages of an LC-MS/MS approach, over more traditional ligand binding assays, is that it does not require specific antibody reagents to achieve adequate selectivity to distinguish between other structurally similar peptides

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The bridge between these two is concentration

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Eight randomized controlled trials (RCTs) involving 31,174 patientsincluding three trials not previously reviewed in meta-analysesand eight observational studies encompassing more than 1.6 million patients were included