The fluorescence spectrum of the lysate of Halo-Rac1-expressing cells, subtracted by that of the non-transfected cells, and the fluorescence spectrum of the SF650T ligand subtracted by that of the sonication buffer alone is shown in Supplementary Fig
Santoro A, Calvo JS, Peris-Daz MD, Krel A, Meloni G, Faller P
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The 10mg mixing chart provides visual references for these concentrations
(29), OVX rats were administered estrogen, progesterone, and a combination of both hormone treatments, with subsequent comparisons regarding changes in activity levels before and after treatment