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Although, most participants lost weight, there was heterogenicity, that has been well-documented in obesity studies [66] and those with higher BMI above 35 experienced greater mean percentage weight loss [64]

Membranes were blocked as earlier in 5% Milk/TBS-T then probed overnight with mouse anti-Catalase primary antibody (Sigma #C0979, diluted 1:1000 in 5% milk/TBS-T) at 4 C then rinsed in TBS-T buffer

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Mitochondrial dysfunction leads to insufficient ATP (adenosine triphosphate) production, affecting the balance of vascular contraction and relaxation