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Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

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Most are Gramnegative and range in function from plant symbionts (e.g., root-associated rhizobacteria) to insect pathogens

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Agents 32 , 405410 (2008)