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Immunohistochemical examination Two series lung tissue of sections taken from each paraffin block and drawn on poly-L-lysine coated slides were stained using recombinant anti-caspase-3 antibody [EPR18297] (ab184787) and recombinant anti-TNF alpha antibody [RM1005] (ab307164) for the expression of TNF- and caspase-3 (cas-3) in accordance with the manufacturers instructions