cerevisiae strains C3G1-C3G10 and DHQC3G1DHQC3G10 (Supplementary Table 5) were restreaked on SC-ULH plates (1.47 g l 1 Synthetic Complete (Kaiser) Drop Out: Leu, His, Ura, 6.7 g l 1 yeast nitrogen base (without amino acids), 20 g l 1 d-(+)-glucose, 16 g l 1 agar) or SC-U plates (SC-ULH, 380 mg l 1 leucine, 76 mg l 1 histidine), respectively
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Kinetic fluorescence measurements were performed in a microplate reader at an excitation wavelength (ex) of 485 nm and an emission wavelength (em) of 530 nm, with a runtime of 15 min at 1-min intervals and a temperature set to 25 C to determine the average velocity (Vmean) of the enzymatic reaction
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Managing stress through meditation or other techniques prevents excessive glutathione depletion
10.1212/01.WNL.0000159743.08996.99 105 FragalaM
