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For the proteomic analysis, 300 L aliquots of urine were centrifuged twice at 10,000 g for 20 min at 4C to separate urinary proteins from small molecules using an Amicon Ultra 0.5 ml Centrifugal Filter (10 kDa, UFC501096, Merck Millipore, Darmstadt, Germany) according to the manufacturers instructions

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In addition, the isoprenyl group can react with cysteine thiol and bind to proteins, thus forming irreversible S -prenylation reactions such as farnesylation

Degeneration pattern of human first-order cochlear neurons