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Endocrine 68:265270 Elshimy G, Chippa V, Correa R (2019) "Myxedema." Engels K, Rakov H, Zwanziger D, Hnes GS, Rehders M, Brix K, Khrle J, Mller LC, Fhrer D (2016) Efficacy of protocols for induction of chronic hyperthyroidism in male and female mice

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Each sample was then incubated with 2 L alkaline phosphatase (0.31 U/L) and 6 L Tris (100 mM, pH 7.5) for 1 h at 37 C