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Method and application to human serum proteins

F.IadecolaC

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Briefly, cells were lysed on ice for 15 min in NETN lysis buffer (20 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM EDTA, 0.5% NP40) supplemented with 10 g/mL aprotinin, 10 g/mL leupeptin, 10 g/mL pepstatin A, 1 mM sodium orthovanadate, 1 mM sodium fluoride, and 1 mM AEBSF (4-[two aminoethyl] benzenesulfonyl fluoride)