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3 Insert the needle at a 4590 angle

Larsen PR

Gaskin RS, Estwick D, Peddi R

Characterizing the biological mechanism(s) by which these molecules achieve weight loss remains a key question in understanding and improving therapeutics for the treatment of obesity

We dont recommend using the cream on a large surface area such as the legs or back since it can sometimes be confusing to patients how large of an area they can safely use this numbing cream on

Plasmid identity was verified using Sanger sequencing, and the most efficient sgRNA (as determined by anti-GCDH immunoblotting) was used in subsequent experiments (sgRNA sequences are listed in Supplementary Table 1)