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Excess crystal violet was removed by washing three times with PBS

It has been shown to exhibit anxiolytic (anti-anxiety) effects without the sedative side effects commonly associated with traditional anxiolytic medications

Local (and systemic) estradiol can stimulate lesion production of PGE2, which can activate pain fibers, enhance neuronal invasion of lesions by stimulating production of NGF and other neurotrophins, and promote sprouting of nociceptors that contribute persistent inflammatory pain and inhibit neuronal apoptosis

It can prevent the risk of anaemia in pregnant women

Retrieved June 26, 2026

Despite initial pharmaceutical interest, AOD 9604 did not progress through the full clinical trial pathway required for regulatory approval, and it holds no Marketing Authorisation (MA) from the MHRA or EMA