Cloning His-tag into CRYAB Construct We characterized the his-tag after cloning it behind CRYAB
The quantification methods include antioxidant assays (e.g., Glutathione assay, Ascorbic acid assay, Antioxidant enzyme activity assay), DNA damage/repair (e.g., 8-OH Guanine, double-strand break assay), peroxidation of lipids (e.g., 4-hydroxynonenal assay, malondialdehyde assay), ROS assay (e.g., H 2 O 2 assay, NO assay), protein modification (e.g., Advanced glycation end products, protein carbonyl assay), and Oxidase/Peroxidase activity (e.g., Monoamine oxidase assay, peroxidase/H 2 O 2 assay)
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As we established, "L-Glutathione" is also a marker of the active form
The expression of several enzymes involved in the mercapturic acid pathway (notably GST and GT) is induced in cancer cells and particularly drug resistant cancer cells ( Table 1 )
This may be beneficial for psoriasis patients and enhance skin health
