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doi: 10.1016/j.mam.2008.05.005

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3T3-L1 preadipocytes were obtained from American Type Cell Collection (ATCC, Manassas, VA, USA), and maintained in DMEM supplemented with 10% calf serum (HyClone, Logan, UT, USA) and 1% penicillinstreptomycin (P/S, Gibco)