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Intracellular reactive oxygen species (ROS) measurement The fluorescent probe DCFH-DA was used to detect the intracellular ROS

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Subsequently, a portion of Fe 2 binds to ferritin heavy chain 1 (FTH1) in the cytoplasm and is oxidized to Fe 3 , then combines with ferritin light chain (FTL) to form ferritin complexes stored within the cell

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This comprehensive approach ensures that patients receive not just immediate relief but also a thorough evaluation of their condition