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Initially, AQP4 knockout mice were employed to determine whether GABA and glutamate participate in AQP4-dependent clearance (Fig
22) Stanley et al
This can result from chronic oxidative stress, heavy metal toxicity (especially iron overload), persistent infections, nutrient insufficiencies (sulfur amino acids, glycine, glutamate, selenium, B-vitamins), liver dysfunction, metabolic stress, and genetic polymorphisms in key enzymes like glutamate-cysteine ligase (GCL) or glutathione S-transferases (GSTs)
Weight loss may be slower, and maintaining results requires more attention to protein intake, resistance training, and metabolic support
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