A stack can test whether broader pathway coverage changes the pattern of results
Figure 9 3.10 Single-cell analysis of macrophage DEGs reveals enrichment of ferroptosis- and autophagy-related pathways in T1MI To further explore the relationship between macrophage-associated immune-inflammatory responses, ferroptosis, and autophagy, we conducted functional annotation and pathway enrichment analysis of differentially expressed genes (DEGs) in macrophages based on single-cell data, following our previous confirmation of ferritinophagy in T1MI ( Figure 10 )
(Etiology) Carnitine-Acylcarnitine Translocase Deficiency Disorder is caused by mutations in the SLC25A20 gene, which provides instructions for making an enzyme called carnitine-acylcarnitine translocase (CACT), which is essential for fatty acid oxidation (a multistep process that breaks down (metabolizes) fats and converts them into energy) Fatty acid oxidation takes place within mitochondria
Indirectly, yes
The math is simple once you understand the relationship between three numbers: the total peptide amount, the volume of water added, and the dose you want
The gene score for each gene was defined as the median log2 fold change in the abundance of each sgRNA targeting that gene
