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Cayman Chemicals), vortexed and EtOH was evaporated using nitrogen stream

Dosing protocols vary based on goals, with monthly costs typically $150-$200 for comprehensive treatment

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MEF cell culture Control, Bmal1 / and Cry1 / Cry2 / MEFs were cultured in DMEM (Sigma-Aldrich) supplemented with 15% FCS (Atlanta Biological) and 1% penicillin-streptomycin at 37 C with 5% CO 2

To obtain additional restraints for structural model determination of the p53 TAD2 -FOXO4 FH complex, we determined paramagnetic relaxation enhancements (PREs) for a set of 7 complexes containing spin-labeled (SL) samples ( 15 N-labeled FOXO4 FH + S46C-SL p53 TAD2 , 15 N-labeled FOXO4 FH + Q52C-SL p53 TAD2 , 15 N-labeled p53 TAD2 + Q112C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + D140C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + N145C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S159C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S162C-SL FOXO4 FH )