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From nine rats of each group, gingivomucosal tissue samples were collected from both the ligature and the non-ligature contralateral sides and vascular permeability was measured as described in Materials and Methods

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34 In summary, unlike in clinical studies, and, although transient biological changes can be detected, disc trauma without loss of nucleus tissue or nucleus herniation and without endplate impairment so far has not shown to promote disc degeneration in biological models

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