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Strategies include testing isolated constituents versus the whole extract, using knockdown of specific pathways in cells or gene knockout models in animals to see if the effect persists, and applying pharmacological inhibitors to block suspected mechanisms

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Otherwise, there is the milder increases of the pressure in the ligated superior sagittal sinus upon additional volume application in the right external jugular vein or in the inferior caval vein, where in the control rats, the portal/caval hypertension remained unchanged, but contributed to the further raise of the increased pressure in the superior sagittal sinus (Figure 5)

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