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The body's ability to absorb B12 from food can diminish with age because stomach acid is required to release B12 from dietary proteins

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Methods for the analysis of vitamin B 12 in food Several methods have been used to determine the vitamin B 12 content in foods including microbiological assays, chemiluminescence assays, polarographic, spectrophotometric, and high-performance liquid chromatography processes

Moreover, our results highlight the essential role of vitamin B12 in homocysteine metabolism, as supplementation was associated with a significant reduction in serum homocysteine levelsan important biomarker associated with cardiovascular and neurodegenerative diseases

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MOTS-c translocates to the nucleus during metabolic stress to regulate antioxidant and glucose metabolism genes via AMPK activation [4]