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If symptoms persist beyond 8 weeks despite optimal hydration and electrolyte management, a 24-hour Holter monitor can quantify ectopic beat burden and guide further intervention

However, it has become clear that the most widely used enzyme-linked immunosorbent assays (ELISA) do not detect zonulin but complement C3 and possibly properdin, a related molecule from the same family with unclear functional effects (73, 74)

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