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The mining of membrane proteins as targets has long focused on extracellular and intracellular regions, and transmembrane domains (TMDs) have been deemed undruggable compared with their water-soluble counterparts

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[PubMed] [Elsevier] Azzini E, Ruggeri S, Polito A

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Pull the plunger back to draw in an amount of air equal to the volume of liquid you plan to withdraw (e.g., pull in 2mL of air if you're going to draw 2mL of water)