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Detection of mitochondrial ROS BHK-21 cells were stained with mitoSOX red mitochondrial superoxide indicator (MCE, Shanghai, China) for 30 min at 37 C in a cell culture incubator

The mass spectrometry proteomics data has been deposited to the ProteomeXchange Consortium ( 2.8 Bioinformatics analysis 2.8.1 LC-MS data analysis SWATH data were processed using Spectronaut v18 (Biognosys AG, Schlieren, Switzerland)

A major role for ferroptosis in Mycobacterium tuberculosis-induced cell death and tissue necrosis