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Homogenates were centrifuged at 800 g for 10 min at 4 C, and the supernatant was sonicated on ice for 20 s before protein quantification (BCA protein assay, Pierce, Rockford, IL, USA)

Fan H, Gong N, Li TF, Ma AN, Wu XY, Wang MW, Wang YX (2015) The non-peptide GLP-1 receptor agonist WB4-24 blocks inflammatory nociception by stimulating beta-endorphin release from spinal microglia

B12 helps support red blood cell production, maintain nerve function, and assist in DNA synthesis

doi: 10.1530/REP-20-0354

If you find that you aren't seeing the results you expect from your routine, it might be worth looking at your gut health

Huang S, Gao Y, Lv Y, Wang Y, Cao Y, Zhao W, et al