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Fluorescence signals were visualized using a fluorescence microscope (Nikon Ts2R) and quantified by flow cytometry (NovoCyte 3000, Agilent, USA)

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At the same concentration of 580 M, Cu(OAc) 2 induced a higher upregulation of IL-1 than CuCl 2 , while CuCl 2 induced a higher upregulation of IL-8, HSPA1A and FOSL1 than Cu(OAc) 2

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When used in conjunction with microneedling, their efficacy skyrockets