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Upon induction of hypoglycemia via an intraperitoneal insulin tolerance test, it was shown that cell-specific RFX6 knockdown pseudoislets secreted less glucagon than controls, however, with global RFX6 knockdown, glucagon secretion was unchanged

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The expression of genes involved in gluconeogenesis, glycogenolysis, lipid synthesis and transport, and catabolism were altered in untreated Hz animals and the expression of most of them was normalized by IGF-1 therapy