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Quantification of absorbance of the blots was performed using densitometric scanning with a Fusion-FX7 system (Vilber Lourmat, Collgien, France) with mean optical density of the samples normalized to that of GAPDH

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The animals were immobilized with a dart gun 39 and a sample of blood was drawn from the jugular vein into EDTA-tubes (S-Monovette K3E, Sarstedt, Hildesheim, Germany) approximately 1530 minutes after darting

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As a control, sterile PBS buffer was used, as in other measurement, with the pH value of 7.2 0.2

[5] Brennan, R., Wells, J