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Regulation of hepatic glutathione metabolism and its role in hepatotoxicity

F., Sanna, P

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Immunocytochemistry Immunolabeling of NPCs and derived neurons was accomplished by utilization of a standard two-day protocol with: 15 min fixation in 4% PFA, 10 min permeabilization in 0.1% triton-PBS and 30 min blocking with 5% donkey serum

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ELISA analysis of the culture supernatants demonstrated that L-carnosine markedly and dose-dependently attenuated the release of IL-1 (Figure 7A) and TNF- (Figure 7B) relative to the LPS-only controls