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Total proteins were extracted with protein extraction buffer (100 mM Tris-HCl pH 8, 150 mM NaCl, 10% glycerol, 5 mM EDTA, 10 mM DTT, 2 mM PMSF, 10 mM NaF, 10 mM Na 2 MoO 4 , 2 mM NaVO 3 , 1%(v/v) NP-40, 1%(v/v) plant protease inhibitor cocktail)

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This is accomplished by activating AMP-activated protein kinase (AMPK) as well as by increasing the translocation of glucose transporter 4 (GLUT4) to the cell membrane

doi: 10.21037/TCR-20-2359 285 FarhanMRizviA

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Differential analysis Differential gene expression analysis was conducted on the dataset using the Limma package in R