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You wont want to expose your protein of interest or the interacting proteins to 20% ethanol, so after adding your beads to a plastic column youll first rinse the beads out with deionized water (dIH 2 O)

After that, 60 g of protein was mixed with sampling buffer and resolved on 7.5% SDSpolyacrylamide gel electrophoresis (PAGE)

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doi: 10.1016/j.phytochem.2022.113459 29 SilbersteinTHar-VardiIHarlevAFrigerMHamouBBaracTet al

10.1016/j.bpobgyn.2016.05.003 79 LongC.FengH.DuanW.ChenX.ZhaoY.LanY.et al (2022)

Correction of riboflavin deficiency in individuals who are both riboflavin and iron deficient improves the response of iron-deficiency anemia to iron therapy (19)