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Additionally, cell viability was evaluated using live/dead staining with a Calcein-AM/PI double staining kit and observed under a fluorescence microscope (Olympus, Japan)

Description Melanotan II (MT-2) is a synthetic analog of the melanocortin peptide hormone alpha-MSH

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Description Reduced glutathione (GSH) is a kind of low molecular scavenger, which can remove O2-, H2O2, LOOH