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Ethics declarations Ethics approval and consent to participate The study was approved by the Ethical Committee of the hospital and informed consent was obtained from the patients or their family who had agreed to participate in the study

Culture media (DMEM/Ham F12 with HEPES and 4.5 g/L glucose), Dulbeccos phosphate-buffered saline (Dulbeccos PBS), Versene, antibiotics, and fetal calf serum (FCS) were purchased from Invitrogen

So well done