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In addition to neuron ferroptosis at the lesion site, SCI causes motor cortex atrophy and functional changes early in the disease, and motor neuron death is thought to be the cause of primary motor cortex atrophy after SCI

The expression levels for each of the genes were normalized to fragments per kilobase of exon model per million mapped reads (FPKM) using a software package called RSEM 79

Cells were grown in LB medium with ampicillin, induced with 0.5 mM IPTG at 25 C for 1012 h, and harvested after cooling to 4 C

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