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Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)

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BPC-157, a synthetic peptide derived from a protective protein found in gastric juice, has demonstrated potent effects on wound healing, vascular growth, and the reduction of systemic inflammation mechanisms that translate directly into improved skin quality when used as part of a topical or subcutaneous protocol