A modified Kolmogorov-Smirnov (K-S) test was applied to the residuals from a replicate-specific accelerated failure time (AFT) model fitted according to the Buckley-James method that uses a nonparametric baseline hazard function
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2-NBDG as a fluorescent indicator for direct glucose uptake measurement
Sterile water contains no preservative, so once you draw from the vial it is single-use and any leftover has to be discarded
they can be dosed precisely, and if kept properly, they will last longer on the shelf
HS-His3MX6-pTEF1-HS and HS-pGSH1-URA3-GFP-tADH1-HS fragments amplified from the relevant pRS315 constructs (described above) were each used to transform both strains BY4741 and BY4742, to replace pGSH1 with pTEF1 and inserting URA3-GFP-tADH1 in the URA3 locus, creating pgsh1 D:: His3MX6-pTEF1