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The samples were desalted using a Zeba spin desalting column (Thermo Fisher Scientific) and 100 l of the desalted sample was combined with 100 l of 100 M NADPH
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In the M-ARCOL model (Mucosal Artificial Colon, an advanced in vitro system using microbiota from healthy donors), daily exposure to PEMPs for 14 days increased pathobiont Desulfovibrionaceae (Fournier et al., 2023)
If needed for a clear medical indication (such as treating pellagra in a pregnant patient), niacinamide can be used with caution, but unnecessary high-dose B supplementation is avoided in pregnancy
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