Electron microscopy sample preparation and data acquisition A 3Ll volume of 5LmgLml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)
The 44 amino acid peptide would be completely hydrolyzed by gastric pepsin and intestinal proteases if swallowed
Boehm A-M, Khalturin K, Anton-Erxleben F, Hemmrich G, Klostermeier UC, Lopez-Quintero JA, et al
[PubMed: 18391525] 324
During the process of renal injury, in addition to the classical abnormal regulation of iron metabolism and lipid peroxidation, a growing number of novel metabolic pathways have also been uncovered in ferroptosis, such as the system Xc - /glutathione peroxidase 4 (GPX4) system, ferroptosis suppressor protein 1 (FSP1)/ubiquinol (CoQ10) system, dihydroorotate dehydrogenase (DHODH)/dihydroubiquione (CoQH 2 ) system, and GTP cyclohydrolase 1 (GCH1)/tetrahydrobiopterin (BH4)/dihydrofolate reductase (DHFR) system ( Figure 1 )
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