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Moreover, smoking causes coronary artery disease and chronic obstructive pulmonary disease, which independently predispose to arrhythmia (DAlessandro 2012)

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As previously described 21 , four microliters (4 g) of reconstituted peptides were loaded and washed on a Pepmap100 C18 trap (5 m, 0.3 5.0 mm, Thermo Fisher Scientific, Waltham, MA, USA) at a flow rate of 20 L/min

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