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2.2 DNA extraction Fecal samples were defrosted and resuspended in 10 mL of phosphate-buffered saline (PBS [pH 7.2]) (containing, per liter, 8 g of NaCl, 0.2 g of KCl, 1.44 g of Na 2 HPO 4 , and 0.24 g of KH 2 PO 4 , all from Sigma-Aldrich) and then centrifuged first at 2,000 rpm at 4 C for 5 min to remove fecal debris and then at 13,000 rpm for 2 min in an Eppendorf 5810R centrifuge to pellet bacterial cells

Soluble fiber from sources like oats, beans, apples, and psyllium husks absorbs water and creates bulk that stimulates bowel movements

Some studies suggest that semaglutide could improve insulin sensitivity

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