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T-REx-SNAP-GLP-1R cells (Fang, Chen, Manchanda, et al ., 2020), generated from parental Flp-In T-REx 293 cells (Thermo Fisher Scientific, MA, USA) by Flp-mediated genomic integration of an insert encoding human GLP-1R with an N-terminal SNAP f -tag and C-terminal SmBiT tag (custom lingen, Germany), with pcDNA5/FRT as the backbone, were maintained in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin