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The perfusion system (UP-100 universal perfusion system, Hugo Sachs Elektronik, Germany) maintained constant flow of 1 ml/min, perfusion buffer was heated and oxygenated (95% O 2 , 5% CO 2 ) and pressure was monitored throughout the experiment (4050 mmHg)

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Oral supplements are available in capsule and liquid forms, though for some conditions, intravenous delivery might be more effective

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