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doi: 10.1016/j.plipres.2022.101165 61

Finally, the slides were fixed with Antifade Mounting Medium (S2100, Solarbio), and images were acquired using a confocal laser scanning microscope (LSM900, ZEISS)

Importantly, an additional experiment showed that LASSS-treated BSA (after the removal of un-reacted LASSS) exhibited nitration resistance to the optimal peroxynitrite-treatment (1:2000 ratio), as shown in supplementary Figure 2, suggesting a model that LASSS-induced chemical modification mechanism exerts a nitration-inhibitory effect on a wide range of proteins susceptible to tyrosine nitration